Supplementary Files
Keywords
Bacterial Cellulose Nanofiber
Biotin-based Aptamer
Cellulose Binding Domain
Streptavidin
How to Cite
Abstract
Recycling is an important approach to preserving the world’s valuable resources for the good and longevity of the planet, as well as to continue to live the way that humans are accustomed to. In this study, a silver ion capture and reusable platform was successfully assembled via a synthetic biology method to recycle metal ions in wastewater. Three modules exist in this platform: (1) a bacterial cellulose nanofiber block (BCNF), which is a structural module; (2) a fusion protein cellulose-binding domain (CBD) and streptavidin (SA)-based linker (CBD-SA-CBD); and (3) a biotin aptamer for metal ion capture blocks. The cellulose BCNF block showed the optimal glucose concentrations for cultivating the bacterial cellulose block. The optimal conditions were identified to induce proteins for the construction of the CBD-SA-CBD linker block. In general, the linker block binds effectively to the metal ion-capturing aptamer, and Aptamers 3 and 4 bind most effectively with silver ions. The reusability of this metal ion-catching aptamer maintained 99.16 to 99.56% binding affinity after four repeated tests. The calculated silver binding efficiency (BE) of the complete assembly remained as high as 99.95%. This consistent binding affinity is a valuable asset in environmental applications. Owing to the diversity of aptamers and versatility of fusion proteins, this genetic engineering design has great potential for diverse future applications, such as heavy metals, antibiotics, pheromones, and even nuclear waste treatment and removal.
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